the effect of vero cell co-culture on the development ofvitrified 8-cell mouse embryos

نویسندگان
چکیده

introduction: it is now generally accepted that vero cells provide beneficial effects on improving embryo quality in many animal species. the aim of this research was to determine the effect of vero cell co-culture on the development of vitrified 8-cell mouse embryos. material and methods: eight- cell mouse embryos were provided from superovulated female nmri mice and divided into treatment case and control groups. in group oftreatment 8-cell mouse embryos were vitrified by 40% ethylene glycol and the thawing was done in 0.5µ sucrose solution. vitrified embryos were transfered to mema medium or mema +vero cells. the control group which consisted of non-vitrified embryos, were also transfered to the same media. the comparison of developmental rate of both groups were evaluated in mema and mema +vero media. results: culture of vitrified embryos in mema medium indicated that there is an increase in the percentage of hatching, while culture of frozen embyros in mema +vero co-culture medium had no effect on the developmental rate of embryos. conclusion: eight-cell stage of mouse embryo is suitable for vitrification and vero cell co-culture is not necessary for improving the development of embryos.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

the co-culture with vero cells and the development of two cell mouse embryos after vitrification

introduction: the mouse embryos can successfully be vitrified using ethylene glycol as cryoprotectant, however their development differs significantly from the non-vitrified embryos. the ability of their development can be improved when they co-culture with somatic cells. in the present study, the effects of co-culturing with vero cells on the development of vitrified two cell mouse embryos wer...

متن کامل

Vero cell co-culture counteracts the detrimental effects of hydrosalpinx fluid on the development of mouse embryos in vitro.

Recent studies have suggested that the hydrosalpinx has a negative effect on pregnancy outcome, with markedly diminished implantation and increased early pregnancy loss. Fluid from the hydrosalpinx may leak into and accumulate in the uterine cavity. It is not clear, however if this creates a hostile local environment in the uterus for embryo implantation or exerts a direct embryotoxic effect. T...

متن کامل

the effect of using visual aids on the development of speech act of disagreement among iranian intermediate efl learners

abstract the present study tried to investigate the effect of visual aids (films) on the development of the speech act of disagreement among iranian efl intermediate learners. to this end, the researcher selected 40 homogeneous intermediate learners based on their scores on oxford placement test. .the subjects then divided into control group and experimental group. both classes were tested by ...

Effects of Visible Light on the Development of Mouse 2-Cell Embryos

Introduction: Mammalian embryos as well as oocytes are prone to various doses of visible light during manipulations in laboratory. The present study was designed to investigate the effects of visible light on the development of mouse 2- cell embryos. Method: Non-pregnant female NMRI mice were super-ovulated with i.p. injection of 7.5 iu PMSG followed 48 hours later with 10 iu hCG. Forty eight h...

متن کامل

the impact of morphological awareness on the vocabulary development of the iranian efl students

this study investigated the impact of explicit instruction of morphemic analysis and synthesis on the vocabulary development of the students. the participants were 90 junior high school students divided into two experimental groups and one control group. morphological awareness techniques (analysis/synthesis) and conventional techniques were used to teach vocabulary in the experimental groups a...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
cell journal

جلد ۵، شماره ۱، صفحات ۱-۷

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023